Tissue, Cartilage RNA Isolation Kit, BioAssay™

RT

Molecular Biology Grade

The Cartilage RNA Isolation Kit contains 4 solutions specifically for RNA isolation from different cartilage tissues. The isolated cartilage total RNA can be used for cDNA library construction, subtracted probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. This kit can also be used for isolation of total RNA from other kinds of tissues.

Kit Components:
T5595-0003A: Solution 1, 1x50ml
T5595-0003B: Phenol A, 1x50ml
T5595-0003C: Solution 2, 1x6ml
T5595-0003D: Solution 3, 1x50ml
T5595-0003E: Solution 4, 1x14ml
T5595-0003F: DEPC H2O/0.1mM EDTA, 1x50ml

Materials Required But Not Supplied:
Isopropanol
100% Ethanol
70% Ethanol
Chloroform

Storage and Stability:
Store T5595-0003A and T5595-0003B at 4°C. Store the rest of the kit components at room temperature. If precipitate forms in T5595-0003E, place the bottle in a 65°C water bath to dissolve it before use. Kit is stable for 6 months.

Quality Control:
Mouse Cartilage total RNA was isolated from each lot:
1.The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA via electrophoreses on a denaturing agarose gel.
2. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5).
.3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.
4. cDNA synthesis is successfully performed by using this RNA as template.