TransScript® Uni Reverse Transcriptase[M-MLV, RNase H-](High Temperature RT)

TransScript® Uni Reverse Transcriptase is a reverse transcriptase with high thermal stability expressed and purified from E. coli after genetic modification and screening. It enables a broard range of reaction temperature (42°C-65°C) with ultra-high thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first-strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of high sensitivity, high specificity, high yield (more full-length cDNA), High thermal stabilityand long half-life.


Features
· Ultra-high thermal stability: reaction temperature 42°C-65°C.
· High sensitivity, high specificity, high highyield and high processivity.
· The unique design of Anchored Oligo (dT)20 can anchor the first base next to the mRNA Poly (A)+5’end, and the binding site is anchored, with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis.
· Deficient RNase H activity. The degradation of the template RNA in the DNA/RNA hybrid in the first-strand cDNA synthesis reaction is avoided, thereby ensuring the yieldand length of the first-strand cDNA.
· Synthesis length can be up to 20kb.


Applications
· High-copy number and low-copy number gene detection.
· High GC or RNA template with complex secondary structure.
· cDNA library construction, primer extension, 3’ and 5’ RACE.


Storage
at -20 ℃ for two years


Shipping
Dry ice (-70 ℃)