Generic 3CLpro FRET peptide substrate - 0.1 mg

Catalog

Peptides

HiLyte™ Fluor 488-QXL® 520

- 20 °C Protected from light

The viral genome of the SARS-CoV-2 virus encodes a main protease, 3CLpro (also called nsp5 or Mpro). The proteolytic activity of 3CLpro is needed for viral replication. This peptide substrate serves as a generic peptide substrate for 3CLpro enzymes based on the site-specific cleavage sequence for many coronavirus 3CLpro enzymes. It can be used to measure the activity of the enzyme. This is a FRET peptide where in its intact form, the fluorescence of HiLyte Fluor 488 is quenched by QXL 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte Fluor 488/QXL 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Immunology and Infectious disease; Cell Signaling / Lyophilized

520

5400535177386

Research use