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3C Protease

3C Protease

Item no.	ANSY-YVV07701-5,000U
Manufacturer	AntibodySystem
Amount	5,000 U
Category	Peptides & Proteins Enzymes
Type	Enzymes
Format	Liquid
Specific against	other
Purity	>95% as determined by SDS-PAGE.
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Available
Specificity	HRV-14
Manufacturer - Applications	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Storage Conditions	Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Molecular Weight	47.46 kDa
Product description	3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Storage buffer	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Endotoxin level	Please contact with the lab for this information.
Note	For research use only.
Expression System	E.coli
Biological Activity	One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.
Protein Fragment	3C Protease is cloned from Human rhinovirus and expressed in E.coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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