MARCKSL1, Recombinant, Human, aa1-195, His-Tag (MARCKS-related Protein, F52, MACMARCKS, MLP, MLP1, MRP)

Blue Ice

21, 9

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 20% glycerol.

MARCKS-related protein, also known as MARCKSL1, is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indicator of PKC activation. Expressed in a variety of tissues with highest levels found in testis and uterus, MARCKSL1 participates in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion. Additionally, MARCKSL1 functions as a regulator of Integrin activation and is thought to regulate Integrin- dependent signal transduction pathways, especially those involved in macrophage spreading.

Recombinant protein corresponding to human MARCKSL1, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~21.9kD (218aa) (MALDI-TOF)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.