TDP2, Recombinant, Human, aa1-362, His-Tag (Tyrosyl-DNA Phosphodiesterase 2, AD022, dJ30M3.3, EAP2, EAPII, RP1-30M3.3, TTRAP)

Blue Ice

43, 3

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol, 1mM DTT.

TDP2 is a member of a superfamily of divalent cation-dependent phosphodiesterases. The protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.

Source:
Recombinant protein corresponding to human TDP2, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~43.3kD (385aa) (MALDI-TOF)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMELGSCL EGGREAAEEE GEPEVKKRRL LCVEFASVAS CDAAVAQCFL AENDWEMERA LNSYFEPPVE ESALERRPET ISEPKTYVDL TNEETTDSTT SKISPSEDTQ QENGSMFSLI TWNIDGLDLN NLSERARGVC SYLALYSPDV IFLQEVIPPY YSYLKKRSSN YEIITGHEEG YFTAIMLKKS RVKLKSQEII PFPSTKMMRN LLCVHVNVSG NELCLMTSHL ESTRGHAAER MNQLKMVLKK MQEAPESATV IFAGDTNLRD REVTRCGGLP NNIVDVWEFL GKPKHCQYTW DTQMNSNLGI TAACKLRFDR IFFRAAAEEG HIIPRSLDLL GLEKLDCGRF PSDHWGLLCN LDIIL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.