FES, Active, Recombinant, Human, His-FLAG-Tag (Tyrosine-protein Kinase Fes/Fps, Feline Sarcoma/Fujinami Avian Sarcoma Oncogene Homolog, Proto-oncogene c-Fes, Proto-oncogene c-Fps, p93c-fes, FPS)

Dry Ice

95

Supplied as a liquid in 40mM Tris-HCl, pH7.5, 110mM sodium chloride, 3mM DTT, 2.2mM potassium chloride, 20% glycerol, 80ug/ml FLAG peptide.

FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases. FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Mutations may activate the FES kinase and thereby contribute to cancer. However, recent data strongly suggests that the c-FES protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer.

Source:
Recombinant corresponding to aa2-822 from human FES, fused to His-tag and Flag-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.

Molecular Weight:
~95kD

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Specific Activity:
~235pmol/min/ug

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.