PDE2A, Recombinant, Rat, His-GST Tag (cGMP-dependent 3',5'-cyclic Phosphodiesterase, Cyclic GMP-stimulated Phosphodiesterase, CGS-PDE, cGSPDE)

Dry Ice

133

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 4mM Glutathione, 20% glycerol, 3mM DTT.

Phosphodiesterase 2A is a cGMP stimulated, cAMP/cGMP dual-specific phosphodiesterase whose hydrolytic activity is stimulated by the presence of cGMP binding to the GAF domain. PDE2A has been implicated in penile erectile dysfunction and in the regulation of fluid and inflammatory cell transit across the endothelial cell barrier (i.e., venous and capillary). PDE2A activity is inhibited by erythro-9-(2-hydroxyl-3-nonyl)adenine (EHNA). At least three isoforms have been reported (PDE2A1, PDE2A2, and PDE2A3).

Source:
Recombinant corresponding to full length rat PDE2A, fused to His-GST-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.

Molecular Weight:
~133kD

Specific Activity:
~2500pmol/min/ug

Unit Definition:
One unit is defined as the amount of enzyme that will convert 1pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.