TNFAIP3, Recombinant, Human, FLAG-Tag (Tumor Necrosis Factor Alpha-induced Protein 3, TNF Alpha-induced Protein 3, OTU Domain-containing Protein 7C, Putative DNA-binding Protein A20, Zinc Finger Protein A20, OTUD7C)

Dry Ice

90, 5

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 0.04% Tween 20, 80ng/ul FLAG peptide, 20% glycerol.

Tumor necrosis factor, alpha-induced protein 3 (TNFAIP3) is a de-ubiquitinating enzyme that contains an N- terminal catalytic domain belonging to the ovarian-tumor superfamily of cysteine proteases (1). TNFAIP3 suppresses apoptosis.It is expressed in various cell types in response to a variety of stimuli that activate the transcription factor NF- kB, including Il-1, CD40 ligand, and PMA (3). TNFAIP3 has been strongly suggested as a key protein involved in tamoxifen resistance, and thus represents both a new breast cancer marker and a promising target for developing new strategies to prevent the emergence of acquired mechanisms of drug resistance in breast cancer (2).

Recombinant protein corresponding to aa2-791 from human TNFAIP3, fused to FLAG-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.

Molecular Weight:
~90.5kD

Specific Activity:
As reported

Assay Conditions:
Reaction was performed in 50mM Tris pH 7.4, 1mM DTT, 0.5mM EDTA, 500nM Ub-AMC, and various amounts of TNFAIP3 Reaction was incubated at 37°C for 30 min. and fluorescent signal was measured at excitation of 360nm, and emission at 460nm.

Applications:
Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.


Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.