UbcH6, Active, Recombinant, Human, His-Tag (UBE2E1, Ubiquitin-conjugating Enzyme E2 E1, UbcH6, Ubiquitin Carrier Protein E1, Ubiquitin-protein Ligase E1)

Dry Ice

22

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 3mM DTT, 20% glycerol.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.

Source:
Recombinant corresponding to aa2-end from human UbcH6, fused to His-tag at N-terminal, expressed in E. coli.

Molecular Weight:
~22kD

Applications:
Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.