ULK, Active, Recombinant, Human, FLAG-Tag (Serine/Threonine-protein Kinase ULK1, Unc-51-like Kinase 1, Autophagy-related Protein 1 Homolog, ATG1, hATG1, KIAA0722)

Blue Ice

113

Supplied as a liquid in 40mM Tris-HCl, pH 7.5, 100mM sodium chloride, 2.2mM potassium chloride, 80ug/ml FLAG peptide, 3mM DTT, 20% glycerol.

ULK1 is a serine/threonine kinase that promotes autophagy signaling (3). It forms a complex with mammalian Atg13 (mAtg13) and FIP200. In this complex, mAtg13 mediates the interaction between FIP200 and ULK1 and is required for the phosphorylation of FIP200 by ULK1 in response to nutrient starvation. mTORC1 associates with the ULK1-mAtg13-FIP200 complex through an interaction between raptor and ULK1, phosphorylating ULK1 and mAtg13 to repress the kinase activity of ULK1, thereby suppressing the initiation of autophagy (4). Moreover, ULK1 is involved in the regulation of neurotransmitter trafficking and axonal growth (5).

Source:
Recombinant corresponding to aa2-end from human ULK1, fused to Flag-tag at N-terminal, expressed in FreeStyle 293-F cells.

Molecular Weight:
~113kD

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.