NDUFA5, Recombinant, Human, aa1-116, His-Tag (NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 5, B13, CI-13kB, CI-13KD-B, NUFM, UQOR13)

Blue Ice

15, 8

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDUFA5, belongs to the complex I NDUFA5 subunit family. The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid.

Source:
Recombinant protein corresponding to aa1-116 from human NDUFA5 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~15.8kD (139aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGS LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI MAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.