NELL1, Recombinant, Mouse, aa17-810 (IDH3GL, Neural EGF-like like Protein 1)

Blue Ice

125165

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 250ul sterile PBS.

NELL1 (neural EGF­like like protein 1) is an ~140kD modular glycoprotein that plays an important role in bone physiology (1). NELL1 contains an N­terminal Laminin G­like domain and three vWF-C domains interspersed with five tandem EGF­like domains (2). Mature mouse NELL1 shares 98% and 93% aa identity with rat and human NELL1, respectively. NELL1 can be retained in the cytosol where it interacts with ARP3 (apoptosis-related protein 3) and becomes phosphorylated by PKC (3, 4). NELL1 is secreted as an ~400kD noncovalent homotrimer of heavily glycosylated subunits (5). It is expressed in bone and in select B cell lines (6, 7). NELL1 promotes the osteogenic differentiation of adipose­derived stromal/stem cells and inhibits adipogenic differentiation (8). It does not promote osteoblastic differentiation from myoblasts, but it does enhance the activity of BMP­2 in that function (9). NELL1 interacts directly with osteoblasts via Integrin alpha3beta1 (10, 11) and promotes osteoblast differentiation and mineralization (3, 12, 13). It synergizes with BMP­2 to increase phosphate uptake through the transporters Pit1 and Pit2 in pre­osteoblasts (14). In vivo, NELL1 expression in the cranium is localized at sites of suture closure (6). Its overexpression induces multiple abnormalities in cranial development including premature suture closure (craniosyntosis) and overlapping sutures (12, 13). Experimental engrafting demonstrates the ability of NELL1 to promote new bone formation and the healing of calvarial defects (13, 15).

Source:
Recombinant protein corresponding to mouse NELL1, Arg17-Asn810, fused to a C-terminal 6xHis tag, CHO-derived.

Molecular Weight:
~125-165kD

Endotoxin:
<0.10EU/1ug (LAL method)

Biological Activity:
Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1-4ug/ml.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.