NAD Kinase, Recombinant, Bacillus subtilis (Probable Inorganic Polyphosphate/ATP-NAD Kinase, Poly(P)/ATP NAD Kinase, PpnK, YjbN, EC 2.7.1.23)

Dry Ice

30

Supplied as a liquid in 50mM TRIS-HCl, pH 7.0, 150mM sodium chloride, 1mM EDTA, 1mM dithiothreitol.

NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus.

Catalytic activity:
ATP + NAD+ = ADP + NADP+.

Source:
Recombinant protein corresponding to full-length Bacillus subtilis NAD kinase, expressed in E. coli.

Molecular Weight:
~30kD

Biological Activity:
~2.6U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.