Deoxyribonuclease I, Recombinant, Human (rhDNase, Dnase)

Blue Ice

37

Supplied as a liquid in 150ug calcium chloride dehydrate, 8.77mg sodium chloride.

Deoxyribonuclease I Human Recombinant (rhDNase) is an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2. Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.

Source:
Recombinant protein corresponding to human Dnase, a glycosylated, polypeptide chain containing 260aa, expressed in CHO cells.

Molecular Weight:
~37kD

Unit Definition:
Dnase is generally assayed according to the photometric method developed by Kunitz (see Kunitz, M., Journal of General Physiology, vol 33, p.349 and 363, 1950). One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1ug of lambda phage DNA in 10 minutes at 37°C in 50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.