Hemagglutinin Influenza A Virus H1N1 H1 (H1N1), Recombinant (A/New Caledonia/20/99) Control for Western Blot

Blue Ice

75

Supplied in denaturing SDS-PAGE sample buffer ready to load on gels. Add 5ul of fresh 2x sample buffer/10ul of the solution prior to heating and loading on gels.

Influenza A (H1N1) virus is a subtype of influenza A virus and was the most common cause of human influenza (flu) in 2009. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a small fraction of all seasonal influenza. H1N1 strains caused a few percent of all human flu infections in 2004–2005. Other strains of H1N1 are endemic in pigs (swine influenza) and in birds (avian influenza). In June 2009, the World Health Organization declared the new strain of swine- origin H1N1 as a pandemic. This strain is often called swine flu by the public media. Swine influenza (also called swine flu, or pig flu) is an infection by any one of several types of swine influenza virus. Swine influenza virus (SIV) is any strain of the influenza family of viruses that is endemic in pigs. As of 2009, the known SIV strains include influenza C and the subtypes of influenza A known as H1N1, H1N2, H3N1, H3N2, and H2N3.

The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function.

Recombinant protein corresponding to full-length Hemagglutinin Influenza A Virus (H1N1), expressed in 293 cell culture infected with H1N1 A/New Caledonia/20/99.

Molecular Weight:
~75kD

Applications:
Suitable for use as a Control for Western Blot. It is not suitable for ELISA or other applications where native protein is required. Other applications not tested.

Recommended Dilution:
Recombinant protein control ~75kD
Heat at 95°C for 2-3 min prior to loading on gels (10ul/lane). SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. This preparation is not biologically active.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.