Hepatitis Virus Spike Protein S1, Recombinant, Mouse (MHV, Coronavirus, MHV-S1)

Blue Ice

45

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies.

Mouse hepatitis virus is a virus of the family Coronaviridae, genus coronavirus. Mouse hepatitis virus (MHV) is a coronavirus that causes an epidemic mouse illness with high mortality, especially among colonies of laboratory mice. Prior to the discovery of SARS-CoV, MHV had been the best-studied coronavirus both in vivo and in vitro as well as at the molecular level. Some strains of MHV cause a progressive demyelinating encephalitis in mice which has been used as a mouse model for multiple sclerosis. Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. The genomic size of coronaviruses ranges from ~26-32kb, extraordinarily large for an RNA virus. The name "coronavirus" is derived from the Latin corona, meaning crown or halo, and refers to the characteristic appearance of virions under electron microscopy (E.M.) with a fringe of large, bulbous surface projections creating an image reminiscent of the solar corona. This morphology is actually formed by the viral spike (S) peplomers, which are proteins that populate the surface of the virus and determine host tropism. Coronaviruses primarily infect the upper respiratory and gastrointestinal tract of mammals and birds. Four to five different currently known strains of coronaviruses infect humans. Proteins that contribute to the overall structure of all coronaviruses are the spike (S), envelope (E), membrane (M) and nucleocapsid (N). MHV diagnosis by serology (histopathology, PCR, IFA or ELISA). MHV spike protein S1 precursor (precursor 1324aa, aa15-1324 mature protein; aa15-717 (S1); 718-1324 (S2). MHV-S1 protein has been used for the diagnosis of MHV infection.

Source:
Recombinant protein corresponding to 391aa from mouse Hepatitis virus, fused to His-tag, expressed in E. coli.

Molecular Weight:
~45kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: load 100-200ng/well
ELISA: 50-100ng antigen/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.