Norovirus 1 Capsid Protein 1, Recombinant, Mouse, His-Tag (MNV-VP1, MNV-1)

Blue Ice

61

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Noroviruses are a genetically diverse group of single-stranded RNA, non enveloped viruses in the Caliciviridae family. The viruses are transmitted by fecally contaminated food or water, by person-to-person contact, and via aerosolization of the virus and subsequent contamination of surfaces. Noroviruses are the most common cause of viral gastroenteritis in humans. Norovirus affects people of all ages. The genus name Norovirus is derived from Norwalk virus, which causes ~90% of epidemic nonbacterial outbreaks of gastroenteritis around the world, and may be responsible for 50% of all foodborne outbreaks of gastroenteritis in the United States.

MNV is closely related to the human Norovirus. Mouse norovirus (MNV), a non-enveloped ss-RNA virus (Calciviridae) and the most prevalent viral infection in laboratory animal facilities, is highly contagious in causing a mild, persistent enteric infection. MNV replicates in macrophages and dendritic cells, with the potential to alter research data. Noroviruses contain a positive- sense RNA genome of ~7.5kbp, encoding a major structural protein (VP1) of about 58~60kD and a minor capsid protein (VP2). The most variable region of the viral capsid is the P2 domain, which contains antigen-presenting sites and carbohydrate-receptor binding regions.
MNV infection may be diagnosed by ELISA, measuring rapidly rising antibody titers (8-12 days after infection) to MNV antigen. Mice infected with MNV are not suitable for animal research; in addition to lung changes, MNV may predispose to secondary bacterial infection, cause infertility, and death in susceptible strains. Besides infecting animals, MNV may also contaminate cell lines, transplantable tumors and other biological products; these should be tested by mouse antibody production (MAP), using ELISA to detect anti-MNV after immunization. A recent study in the USA found approx. 22% mouse samples were positive for MNV.

Source:
Recombinant protein corresponding to 541aa from full-length mouse MNV-VP1, fused to His-tag, expressed in E. coli.

Molecular Weight:
~61kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.