Polyomavirus Capsid Protein 1, Recombinant, Mouse, His-Tag (KVP-VP1)

Blue Ice

65

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies.

Mouse polyomavirus a member of the family Papovaviridae. It is a dsDNA virus. It has been shown to cause asymptomatic infections in laboratory and wild mice. The virus is one of the few tumor viruses amendable to study in its natural host. When inoculated as newborns,
mice of certain genetic backgrounds develop an array of solid tumors arising from at least a dozen distinct cell types. By 3-4 months of age, individual animals typically bear tumors of multiple types and with a combined tumor mass that may approach 25% or more of total body weight. PyV is antigenically different from the K virus (aka pneumotropic Kilham strain). The viruses are distinct from each other after examination with six different restriction endonucleases which revealed no similarities between both DNAs. Studies indicate there is no homology between PyV and the Kilham mouse polyoma virus. Mouse polyoma virus KV strain (mouse pnuemotropic virus or myotis polyoma virus) VP1 (373aa) is highly antigenic protein.

Source:
Recombinant protein corresponding to 373aa from full-length mouse Polyomavirus Capsid Protein 1, fused to His-tag, expressed in E. coli.

Molecular Weight:
~65kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.