Sialodacryoadenitis Virus Nucleoprotein, Recombinant, Rat, aa17-440, His-Tag (SDAV-NP)

Blue Ice

49, 5

Supplied as a liquid in 50mM Tris, pH 8.0, 0.25M sodium chloride, 5mM beta- mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies.

Sialodacryoadenitis virus (SDAV) is distributed worldwide in laboratory rats. SDAV infects the lacrimal and salivary glands and the upper and lower respiratory tracts of rats, causing the clinical manifestations of enlarged salivary glands, sialoadenitis, dacryoadenitis, rhinitis, tracheitis, and bronchoalveolitis. SDAV can also cause reproductive disorders and behavioral changes in the infected animals. Serologic surveys indicate that coronavirus infections are common in laboratory rats housed in research facilities and several outbreaks of SDAV in rat colonies have been reported. Therefore, SDAV is an important viral pathogen in comparative laboratory medicine. SDAV is antigenically related to the mouse hepatitis virus (MHV) serogroup of the family Coronaviridae in the order of Nidovirales. The MHV serogroup includes Parker’s rat coronavirus (PRCV), bovine coronavirus (BCV), and human coronavirus (HCV) strain OC43. As with mouse hepatitis coronaviruses (MHVs), the SDAV genome was code for at least three structural proteins associated with the virion: spike (S) protein, membrane (M) protein, and nucleocapsid (N) protein. Antibodies specific for MHV structural proteins were able to recognize both SDAV and PRCV proteins on immunoblots.

Source:
Recombinant protein corresponding to full-length rat SDAV-NP, aa17-440, fused to His-tag, expressed in E. coli.

Molecular Weight:
~49.5kD

Applications:
Suitable for use in ELISA, Western Blot or other applications where native protein is required. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.