Toolan's H1 Parvovirus, Capsid Protein VP2, Recombinant, His-Tag (THV-VP2)

Blue Ice

81

Supplied as a liquid in 50mM Tris, pH 8.0, 0.25M sodium chloride, 5mM beta- mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies. Parvovirus casually applied to all the viruses in the Parvoviridae taxonomic family and also the taxonomic name of the Parvovirus genus within the Parvoviridae family. Parvoviruses (from Latin parvus meaning small) are typically linear, non-segmented single-stranded DNA viruses, with an average genome size of 5Kb. Parvoviruses tend to be specific about the taxon of animal they will infect. The viral capsid of a parvovirus is made up of two or three proteins, known as VP1-3 that form an icosahedral structure that is resistant to acids, bases, solvents and temperature up to 50°C. Structural protein (NS1-2) are conserved and involved in transcription and virus replication. Capsid proteins (VP1-3) exhibit heterogeneity among different parvoviruses. Parvovirus diagnosis is by serology and ELISA. MPV is most pathogenic for haematopoietic cells than mouse parvoviruses (MPVs). Rat Toolan’s H-1 (H-1/or THV) is a member of parvovirus family and closely related to the minute virus of mice (MVM). The clinical signs associated with a natural THV infection lead to cell death.

Species: Minute virus of mouse (MVM) or mice minute virus (MMV), Kilham rat virus (KRV), Rat H-1 virus (Toolan’s virus), mouse parvovirus (MPV), hamster (HaPV) and rat parvovirus (RPV- 1a).
Natural hosts: Vertebrates.
Transmission: Respiratory, oral droplets of fecal oral-route.
Geography: Worldwide.

Source:
Recombinant protein corresponding to 592aa from full-length THV-VP2, fused to His-tag, expressed in E. coli.

Molecular Weight:
~81kD

Specificity:
THV-VP2 protein has significant sequence homology with related member of parvoviruses (rat kilham virus VP2, ~80%, rat and mouse minute viruses VP2 ~72%).

Applications:
Suitable for use in ELISA, Western Blot or other applications where native protein is required. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.