DNMT3L, Recombinant, Human, aa1-386, His-Tag (DNA (Cytosine-5)-Methyltransferase 3-Like Isoform)

Blue Ice

46, 3

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 10% glycerol.

DNA (cytosine-5)-methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints.

Source:
Recombinant protein corresponding to aa1-386 from human DNMT3L, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~46.3kD (412aa), confirmed by MALDI-TOF

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.