Adenovirus, Strain Adenoid 6

Dry Ice

30

Supplied as a liquid in MEM. No preservative added.

Adenoviridae family. A non enveloped, icosahedral virion, 75-80 nm diameter, double-stranded, linear DNA genome. Varies in clinical manifestation and severity. Symptoms include fever, rhinitis, pharyngitis, cough and conjunctivitis.

Contains high concentration of virus and viral components as well as some cellular material.

Source:
Adenovirus, Strain Adenoid 6, expressed in MRC-5 cells

Inactivation:
Gamma radiation.

Purification:
Optimally infected cells are disrupted by sonication and then subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated by cross flow ultrafiltration with a 30, 000 molecular weight cut-off.

Applications:
Suitable for use in ELISA with polystyrene and latex solid phases for detection of both IgG and IgM. Other applications not tested.

Recommended Dilution:
The antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. This preparation may be further purified to meet the needs of a particular assay format.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.