Collagenase ≥ 300 u/mg, C. histolyticum

Molecular Biology / MB-Enzymes

4°C

Supplied as a lyophilized powder.

Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.

Collagenase Nomenclature:
Type 1-Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells.
Type 2-Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells.
Type 3-Containing low proteolytic activity. It is usually used for mammary and fetal cells.
Type 4-Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.

Absorbance:
As reported

Activity:
≥ 500 units/mg dry weight (dw)
≤ 50 units/mg Caseinase (dw)
≤ 2.0 units/mg Clostripain (dw)
≤ 0.25 units/mg Tryptic (dw)

Unit Definition:
1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.

Units per Vial (10Ku):
≥10, 000 units/vial

Endotoxin EU/mg dw:
As Reported

Quality Control:
SDS-PAGE

Storage and Stability:
Collagenase is stable for 6 months at 4ºC.