Cytomegalovirus, Strain AD 169 [MRC-5] (CMV)

Molecular Biology / MB-Infectious Disease

-70°C

Supplied as a liquid in glycine buffered saline, pH 9.5. Sonicate immediately before use to ensure the preparation is uniform.

Cytomegalovirus is a member of the Herpesviridae family. It may be transmitted from person to person through urine, saliva, other bodily fluids, breast milk and transplanted organs. It is a common infection which mostly passes unnoticed. The infection may be dangerous to people who are immunocompromised such as those who are HIV positive or organ transplant recipients. The infection may also be dangerous to pre- and post-natal infants. Antiviral drugs are useful in the treatment of CMV infection.

Cytomegalovirus strain AD-169, cultured in MRC-5, a diploid cell line of human lung origin. MRC-5 monolayers are infected in a manner which will ensure that the full host of viral proteins are present upon harvest. Optimally infected tissue is collected in glycine buffered saline. The cells are disrupted to release cellular CMV antigen and cellular debris is removed by centrifugation. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Activity:
Antigenic activity is 112% of internal reference standard.

Inactivation:
gamma radiation, which primarily damages viral genetic material.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.