β-Galactosidase, Recombinant, E. coli

Blue Ice

540

Supplied as a lyophilized powder in PBS, 74% sucrose. Reconstitute with 1ml sterile ddH2O.

E. coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.

Source:
Purified protein from E. coli

Molecular Weight:
~540kD

Specific Activity:
> 600U/mg protein.

Unit Definition:
One unit hydrolyses 1umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.

Protein Content:
~20-25% of total weight

Applications:
Suitable for use in Functional Assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.