Glutathione S-Transferase Yb2, Rat (GST)

Molecular Biology / MB-BioSep Assay Detection, Tags

-70°C

Suspended in 20mM Tris buffer (pH 7.0).

Each vial contains sufficient enzyme for 2 assays using CDNB as substrate in a spectrophotometric assay.

Source:
Rat Liver

Specific Activity:
5.9units/mg

Applications:
Suitable for use as a standard for Western blotting or for ELISAs. Other applications not tested.
Optimum dilutions to be determined by researcher.

Definition of unit:
One unit is the amount of enzyme that catalyzes the conjugation of 1uM of 1-chloro-2, 4-dinitrobenzene (CDNB) to glutathione in one minute at room temperature in 100mM potassium phosphate buffer, pH 6.5. [J.B.C., 249 : 7130-7139 (1974)]

Storage and Stability:
Stable indefinitely at -70°C. Labile to repeated freezing and thawing.