Griffonia (Bandeiraea) simplicifolia GS I (GS-I) (AP)

Molecular Biology / MB-Lectins

4°C Do Not Freeze

Supplied as a liquid in 0.01M PBS, 0.15M sodium chloride, 0.5mM calcium chloride, 2mM Melibiose, pH 7.2-7.4, 20-50% glycerol. Labeled with Alkaline Phosphatase (AP).

Pure Griffonia conjugated. simplicifolia lectin (GS- I), Alkaline Phosphatase conjugated.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity: Melibiose, alpha-D-Galactose

Inhibitory Carbohydrate: alpha-Galactose

Activity: 20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activity against all blood types increases after neuraminidase treatment of the cells.

Chemical Used for Conjugation: Alkaline Phosphatase.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.