HIV-1 gp160, Soluble, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold)

Molecular Biology / MB-Infectious Disease

4°C Do Not Freeze

Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Labeled with Colloidal Gold.

rgp160 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).

Coupling Ratio:
OD520nm = 10; protein content approximately 25ug.

Specificity:
Gold conjugated rgp160 binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.

Applications:
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase CD4/Anti-gp160 in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.