MGMT, Recombinant, Human, active (O6-Methylguanine DNA Methyltransferase)

Molecular Biology / MB-Enzymes, Methyltransferase

-20°C

Supplied as a liquid in 20mM Tris, pH 7.5, 1mM DTT, 1mM EDTA, 200mM sodium chloride, 50% glycerol.

Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-Methylguanine DNA Methyltransferase) removes alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to its cysteine residue, thereby irreversibly inactivating MGMT. Inactivated MGMT is rapidly degraded. Therefore, MGMT levels serves as an indicator of DNA damage.
Tumors with high level of MGMT are likely to be drug resistant and low or no MGMT may make the tumor more responsive to chemotherapy. In normal cells, Abnormally low level of MGMT may make normal cells/tissue more susceptible to tumor promoting/alkylating agents.

Specific Activity:
Minimum 10, 000 Units/mg protein. One unit is defined as the amount of protein that cleaves 50% of 0.5 pmole of dsDNA oligomer substrate (O6-methylguanine) in 10 min at 37°C.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.