MMAB, aa33-250, Recombinant, Human (Cob(I)yrinic Acid a,c-diamide Adenosyltransferase, Mitochondrial, Cob(I)alamin Adenosyltransferase, Methylmalonic Aciduria Type B Protein)

Blue Ice

26, 3

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 10% glycerol.

MMAB is a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase(MCM). Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group.

Source:
Recombinant corresponding to aa33-250 of human MMAB protein, fused to His-tag at N-terminus, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MQSRGPQGVE DGDRPQPSSK TPRIPKIYTK TGDKGFSSTF TGERRPKDDQ VFEAVGTTDE LSSAIGFALE LVTEKGHTFA EELQKIQCTL QDVGSALATP CSSAREAHLK YTTFKAGPIL ELEQWIDKYT SQLPPLTAFI LPSGGKISSA LHFCRAVCRR AERRVVPLVQ MGETDANVAK FLNRLSDYLF TLARYAAMKE GNQEKIYKKN DPSAESEGL

Molecular Weight: 26.3kD (239aa), confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.