NFkB Activating Kinase, CT, Blocking Peptide (Nuclear Factor kappa B, NAK, TBK1, TANK-Binding Kinase 1)

Molecular Biology / MB-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Synthetic peptide (ERFGSLTMDGGLRNVD) corresponding to amino acids 712 to 727 of human NAK/TBK1 (1, 2), which are identical to those of mouse homologue (1).

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. Phosphorylation of I-kB by IkB kinase (IKK) complex leads to degradation of I-kB and activation of NF-kB. The IKK complex contains IKKa, IKKb, and IKKg. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKb through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1, 2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.