NIK, CT, Blocking Peptide (NF-kappa-beta-inducing Kinase, HsNIK, FTDCR1B, HS, Mitogen-activated Protein Kinase Kinase Kinase 14, MAP3K14, Serine/threonine Protein Kinase NIK)

Molecular Biology / MB-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Peptide corresponding to aa931-947 of human NIK.

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, LPS and mitogens. A serine/threonine protein kinase which mediates NF-kB activation by IL-1, TNFa and CD95 was identified recently and designated NIK (for NF-kB inducing kinase). NIK is an activator of IkB kinase alpha and beta (IKKa and IKKb). Therefore, NIK is a key molecule in the NF-kB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The b-chemokines RANTES, MIP-1a and MIP-1b are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.