Norepinephrine Transporter, Rat Control Peptide (NET)

Molecular Biology / MB-Ion Channel

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium merthiolate.

Control Peptide for N3950-05 (antiserum) and N3950-05A (affinity purified)

15 mer peptide mapping to the 1st extracellular domain of NET. 100% homology to mouse, rat and 90% homology to human and bovine NET. The peptide shows no significant homology with other known proteins.

Noradrenaline transporter or epinephrine transporter (NET or NTT1 or NAT1 or SLC6A2) gene (chromosome 16) encodes a protein of 617aa (mouse 617aa, ~94% identity), with 12-13 transmembrane domains, and cytoplasmic N and C-termini. Expression of the cDNA clone in transfected HeLa cells indicated that noradrenaline transport activity is sodium-dependent and sensitive to selective noradrenaline transport inhibitors. NET mRNA has been localized to the brain stem and adrenal. The predicted protein sequence demonstrated significant amino acid identity with the Na(+)/gamma-aminobutyric acid transporter, thus identifying a new gene family for neurotransmitter transport proteins. Alternative splicing of NET mRNA produces two transporter isoforms, rNETa and rNETb, which differ at their COOH termini. The rNETa isoform reveals a COOH terminus homologous to human NET and transports norepinephrine. In contrast, rNETb revealed no detectable transport function, but reduced functional expression of rNETa when both isoforms were expressed in the same cell. Defects in NET are a cause of orthostatic intolerance (OI), a syndrome characterized by lightheadedness, fatigue, altered mentation and syncore. It is associated with postural tachycardia.

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.

Recommended Dilution:
Antibody Blocking: 5-10ug per 1ul N3950-05 (antiserum) or per 1ug N3950-05A(affinity purified antibody).
ELISA: 50-100ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.