NQO1, Recombinant Human (NAD(P)H Dehydrogenase Quinone 1)

Molecular Biology / MB-Enzymes, Dehydrogenase

-20°C/-70°C

Supplied as liquid in 20mM Tris-HCL buffer, pH 8.0, 10% glycerol, 1mM DTT

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer’s disease (AD). Recombinant NQO1 protein , expressed in E. coli and purified by using conventional chromatography techniques.

Source:
Recombinant human NQO1, aa1-274, His-tagged, expressed in E. coli

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.