Nucleoside Diphosphate Kinase D, Recombinant, Human, aa33-187, His-Tag (NDPKD, NDPK-D, NM23D, NM23H4, nm23-H4, NME4, Nucleoside Diphosphate Kinase Mitochondrial, NDP Kinase Mitochondrial, NDK)

Blue Ice

19, 6

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 40% glycerol.

NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP.

Source:
Recombinant corresponding to aa33-187 from human NME4, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Molecular Weight:
~19.6kD (176aa) confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.