PDXK, aa1- 312, Recombinant, Human (Pyridoxal Kinase, Pyridoxine Kinase, C21orf124, C21orf97, PKH, PNK, PRED79)

Blue Ice

37, 6

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.

PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism ofaas and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Source:
Recombinant corresponding to aa1-312 of human PDXK protein, fused to His-tag at N-terminus, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAEL LSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL

Molecular Weight: 37.6kD (336aa) confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.