Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (Agarose)

Blue Ice

128

Supplied as a suspension in PBS, pH 7.2, sodium azide. Agarose coupled. Bead Size: 50-250um. Spacer and Bead Linkage: Imidoester. Spacer: None.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. Thesy can be used to select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity:
Recognizes a complex branched chain oligosaccharide structure.

Inhibitory Carbohydrate:
Not inhibited by simple sugars.

Carbohydrate for Elusion:
0.1M Glycine pH 3.5-4.0. Simple sugars are not effective for elution.

Activity:
≤ 5ug/ml will agglutinate Type O human erythrocytes.
≤ 0.5mg/ml will agglutinate neuraminidase-treated erythrocytes.

Application(s):
Suitable for use in Western Blot, Dot Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Also Available:
P3370: Phaseolus vulgaris E (PHA-E, Red Kidney Bean)
P3370-10: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (Agarose)
P3370-11: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (Biotin)
P3370-20: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (FITC)
P3370-25: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (HRP)