Phaseolus vulgaris L (PHA-L, Red Kidney Bean)

Blue Ice

128

Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with 2ml sterile buffer.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates. They are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity:
Recognizes a complex branched chain oligosaccharide structure.

Inhibitory Carbohydrate:
Not inhibited by simple sugars

Activity:
A minimum of 1mg/ml is required to agglutinate Type O human erythrocytes. Less than 0.5ug/ml will agglutinate neuraminidase-treated cells.

Quality Control:
SDS-PAGE

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.