Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (FITC)

Blue Ice

128

Supplied as a liquid in 0.02M sodium bicarbonate, pH 9.0-9.5, 0.05% sodium azide. Labeled with FITC. FITC: Protein (OD495/OD280) = 2.28

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. Thesy can be used to select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Specificity:
Recognizes a complex branched oligosaccharide structure. Not inhibited by simple sugars.

Activity:
≤ 5ug/ml will agglutinate Type O human erythrocytes.
≤ 0.5mg/ml will agglutinate neuraminidase-treated erythrocytes.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.