Phosphodiesterase 2A, Recombinant, Human (PDE2A)

Dry Ice

132

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol and 3mM DTT.

Mouse PDE2A (GenBank Accession No. NM_002599), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specific Activity:
2080pmol/min/ug

Assay Conditions:
10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cGMP, 2.5kU 5’nucleotidase; 37°C, 20 min.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.