Phosphodiesterase 5A, Recombinant, Human, GST-Tag (PDE5A)

Dry Ice

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Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 0.04% Tween 20, 3mM DTT, 20% glycerol.

Human PDE5A (GenBank Accession No. NM_001083), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specific Activity:
~3000 pmol/min/ug

Activity:
Quantified by 5’-nucleotidase cleaving the 5’GMP product and releasing the phosphate group which was detected by Malachite Green Reagent.

Unit Definition:
One unit is defined as the amount of P4072-05A that will convert 1pmole of 3’, 5’-cGMP to 5’-GMP per min at 37ºC under Assay conditions.

Assay Conditions:
10mM Tris-HCl, pH 7.4, 10mM MgCl2, 200uM cGMP, 0.1mg/ml BSA, 0.05% Tween-20, 200uM cGMP and serial dilutions of PDE5A at 37ºC for 20 minutes.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.