Phosphodiesterase 9A2, Recombinant, Human, GST-Tag (PDE9A2)

Dry Ice

87, 7

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 5mM glutathione, 3mM DTT, 30% glycerol.

Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specific Activity:
~90U/ug. 1 unit will lease 1 pmole phosphate per minute from 400uM 5'- GMP at 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 1mM MgCl2.

Assay Buffer:
10mM Tris-HCl, pH 7.4, 1mM MgCl2; 0.4mM 3', 5'-cGMP substrate, 5ng/ul
PDE9A.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.