Phosphodiesterase 10A, Recombinant, Human, GST-Tag (PDE10A)

Dry Ice

116

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2 mM KCl, 3mM DTT, 0.04% Tween-20, 20% glycerol.

Full length recombinant corresponding to human PDE10A with an N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Accession No. NM_006661.

Specific Activity:
As reported

Unit Definition:
1 unit is the amount of PDE10A that will convert 1 pmole 3’, 5’-cAMP to 5’-AMP per minute at 37°C.

Assay Conditions:
10mM Tris-HCl, pH 7.4, 10mM MgCl2; 0.1mg/ml BSA, 0.05% Tween-20, 200uM cAMP and serial dilutions of PDE10A at 37ºC for 20 min. Quantified by 5 nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Avoid repeated freezing and thawing. Store at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.