Phospholipase A2

Blue Ice

30000

Supplied as a lyophilized powder

Phospholipase A2 is a member of the class of heat-stable, calcium-dependent enzymes catalyzing the hydrolysis of the 2-acyl bond of 3-n-phosphoglycerides. The enzyme has a molecular weight of 30, 000 daltons. Phospholipase A2 is activated by Ca2+. It is inhibited by zinc, barium, and manganese ions. Activity values for phospholipase A preparations which are derived from titrimetric assay procedures can be quite dependent on source and type of lecithin, its preparation as a substrate emulsion, other components of the reaction mixture, and the method and instrumentation used.

Activity:
≥200 units per mg dry weight

A280 @mg/ml:
As Reported

Unit Definition:
1 unit releases 1umole of acid from soybean lecithin per minute at 25°C, pH 8.9

Activators:
Calcium ion (Dennis 1973)

Inhibitors:
Zinc, barium and manganese ions (Uthe 1971). Also see Golec et al. (1992).

Isoelectric Point:
4.55 and 4.40 for Aalpha and Abeta, respectively

Extinction Coefficient:
As Reported

Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.