RhoC, aa1-190, Recombinant, Human (Rho-related GTP-binding protein RhoC)

Blue Ice

23, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.

RhoC, also known as rho-related GTP-binding protein RhoC, is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. RhoC promotes reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility.

Source:
Recombinant corresponding to aa1-190 of human RhoC protein, fused to His-tag at N-terminus, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR
PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS
AKTKEGVREV FEMATRAGLQ VRKNKRRRGC

Molecular Weight: 23.8kD (210aa) confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.