RhoB, Recombinant, Human, aa1-193, His-Tag (ARH6, ARHB, RHOH6, Ras homolog family member B, ARH6, Aplysia Ras-related homolog 6, ARHB, RhoH6)

Blue Ice

23, 9

Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 2mM DTT, 0.2M sodium chloride, 20% glycerol.

Rho-related GTP-binding protein RhoB, also known as RhoB, is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation.

Source:
Recombinant corresponding to aa1-193 from human RhoB, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS
AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC

Molecular Weight:
~23.9kD (213aa), confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.