RhoD, Recombinant, Human, aa18-207, His-Tag (ARHD, RHOHP1, RHOM)

Blue Ice

23, 8

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 5mM DTT, 200mM sodium chloride, 2mM EDTA, 0.1mM PMSF, 50% glycerol.

Rho-related GTP-binding protein, also known as RhoD, belongs to the small GTPase superfamily. The small GTPase Rho D contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution.

Source:
Recombinant corresponding to aa18-207 from human RhoD, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH
DNVHAVFQEA AEVALSSRGR NFWRRITQGF C

Molecular Weight:
~23.8kD (211aa), confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.