SAPK4, p38 delta, Active, Recombinant, Human

Blue Ice

71

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, and 50% glycerol.

Recombinant GST fusion protein fused to full length human SAPK4, expressed in E. coli.

Human stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4.

Specific Activity:
~100U/mg

Unit Definition:
1unit pf p38 delta/SAPK4 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein/minute at 30°C with a final ATP of 100uM.

Kinase Assay:
Enzyme was tested in a functional kinase assay by using 10-100ng of active SAPK4 to phosphorylate MBP in vitro.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.