SGK, active, delta 1-60, S422D, Human (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)

Dry Ice

48

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.15M sodium chloride, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 270mM sucrose.

This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.

An N-truncated (missing N-terminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation, expressed in Sf21 cells.

Specific Activity:
~1500U/mg

Unit Definition:
One unit of SGK1 activity is defined as 1nmol phosphate incorporated into Crosstide substrate peptide in one minute.

Quality Control Testing:
MS Tryptic Fingerprint: Confirmed identity as SGK1 with 33% amino acid coverage.

Kinase Assay:
10-100ng phosphorylated Akt/SGK Substrate Peptide in the assay described.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.